Liquid Trypsin
General Description: Trypsin is a proteolytic enzyme that catalyses the hydrolysis of proteins to form smaller polypeptide units. It is produced in the pancreas of invertebrates as the inactive proenzyme trypsinogen. When converted to its active form, trypsin cleaves peptide bonds on the carboxyl side of the amino acids arginine, lysine and s-aminoethyl cysteine. There is little or no cleavage at arginyl-proline or lysyl-proline bonds.
Bovogen Biologicals Trypsin products are extracted from porcine pancreatin using a combination of isoelecric precipitation, salt precipitation, microfiltration and ultrafiltration. The purified product is lyophilised in the presence of a plant derived excipient which provides superior solubility and stability.
Liquid Trypsin is manufactured by reconstitution of lyophilised Trypsin to a variety of formulations, followed by sterile filtration to 0.22 micron and then frozen storage. Product formulations are consistent with specific customer requirements or specifications.
Bovogen Biologicals’ Bovogen Biologicals' Liquid Trypsin is an ideal reagent for both industrial and research applications where the handling of powdered products is considered hazardous or cumbersome.
Raw Material Origin: The raw materials of animal origin used in the manufacture of Bovogen Biologicals’ trypsin are sourced from porcine pancreatic glands of Australian animals which have been veterinary ante- and post-mortem inspected and approved for human consumption.
Appearance: Clear Liquid.
Applications: Applications for liquid porcine trypsin include:
- Resuspension of adherent cells in tissue culture
- Dissociation of dissected tissues or cells
- Cleavage of fractions in vaccine production
- Hydrolysis of proteins used in food and dairy products
Documentation: A Certificate of Analysis is supplied with all orders. A Certificate of Origin is also supplied for export orders.
Storage and Handling: Product is shipped on dry ice with a recommended storage temperature of ≤ -20°C.
Ordering Information: Information on request.
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